Human IL-29/IFN-lambda 1 ELISA Kit (Colorimetric) Summary
15.6 – 1,000 pg/mL
2.0 pg/mL
Sandwich ELISA
7.1%
7.6%
Please See Protocol for Specific Spike Recovery Details
50 uL
ELISA Kit (Colorimetric)
IFNL1
Applications/Dilutions
The human IL-29 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-29
Reactivity Notes
Human
Packaging, Storage & Formulations
Storage of components varies. See protocol for specific instructions.
Kit Components
- Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-29
- Adhesive Films
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Human IL-29 Standard lyophilized, 2 ng/ml upon reconstitution
- Biotin-Conjugate anti-human IL-29 monoclonal antibody
- Sample Diluent
- Stop Solution (1M Phosphoric acid)
- Substrate Solution (tetramethyl-benzidine)
- Streptavidin-HRP
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Alternate Names for Human IL-29/IFN-lambda 1 ELISA Kit (Colorimetric)
- cytokine Zcyto21
- IFNL1
- IFN-lambda 1
- IFN-lambda-1
- IL29
- IL-29
- interferon lambda-1
- interferon, lambda 1
- interleukin 29 (interferon, lambda 1)
- interleukin-29
- ZCYTO21
Background
IL-29 is a protein of the helical cytokine family and is a type III interferon sharing many functions with the type I family of interferons. The IL-29 gene is found on chromosome 19 in humans. It is also known as IFN-lambda1 and is highly similar in amino acid sequence to the IL-28, the other type III interferon. IL-29 does not bind the IFN-alpha/beta receptor, but instead signals through a receptor composed of the IL-28R1 and IL-10R2 subunits. Initial characterizations of the receptor demonstrated that it has a ubiquitous tissue distribution and is expressed on most non-hematopoietic cells. Generation of native IL-29 is achieved by monocytes and dendritic cells in response to viral infection and stimulation with toll-like receptor ligands. IL-29 plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses. IL 29 has significant antiviral activity and immunoregulatory properties and appears to inhibit T helper-2 (Th2) responses regarding inhibition of IL-13 production, compared with IL-4 or IL-5. The antiviral activities of IL-29 include the upregulation of MHC Class I expression on the cell surface and the expression of PKR. The ligand/receptor complex seems to signal through the Jak-STAT pathway.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.