Human IL-17/IL-17A ELISA Kit (Colorimetric) Summary
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into serum. There was no crossreactivity detected.
1.6 – 100 pg/mL
0.5 pg/mL
Sandwich ELISA
9.1%
7.1%
The overall mean recovery in serum samples was107.0%, in plasma (heparin) samples 50.8%, in plasma (citrate) 75.5% and in cell culture supernatant samples 113.6%.
50 ul
ELISA Kit (Colorimetric)
IL17A
Reactivity Notes
Human
Packaging, Storage & Formulations
Storage of components varies. See protocol for specific instructions.
Kit Components
- Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-17A
- Adhesive Films
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Biotin-Conjugate anti-human IL-17A monoclonal antibody
- Human IL-17A Standard lyophilized, 200 pg/mL upon reconstitution
- Control low, lyophilized
- Control high, lyophilized
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
- Streptavidin-HRP
- Substrate Solution (tetramethyl-benzidine)
- Stop Solution (1M Phosphoric acid)
- Sample Diluent
Alternate Names for Human IL-17/IL-17A ELISA Kit (Colorimetric)
- CTLA-8
- CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
- Cytotoxic T-lymphocyte-associated antigen 8
- IL17
- IL-17
- IL17A
- IL-17A
- IL-17Acytotoxic T-lymphocyte-associated protein 8
- IL-17CTLA-8
- IL17interleukin-17A
- interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
- interleukin 17A
Background
The T help 1 (Th1) and Th2 cell classification has provided the framework for understanding CD4(+) T cell biology and the interplay between innate and adaptive immunity. Studies have defined an novel arm of the CD4(+) T cell effector response, the Th17 lineage. IL-17A, a homodimeric cytokine of about 32 kDa, is largely produced by activated memory T lymphocytes, but stimulates innate immunity and host defense. The closest associate to IL-17A is IL-17F, which is encoded in a syntenic fashion and shares around 50% sequence homology. IL-17A and IL-17F both mobilize neutrophils partly through granulopoeisis and CXC chemokine induction. Increased levels of IL-17 have been associated with several conditions, including airway inflammation, rheumatoid arthritis, intraperitoneal abscesses and adhesions, inflammatory bowel disease, allograft rejection, psoriasis, cancer and multiple sclerosis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
4-Deazaneplanocin A (hydrochloride)