Human CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric) Summary
0.16 – 10.0 ng/mL
0.06 ng/mL
Sandwich ELISA
6.80%
4.00%
91%
20 uL
ELISA Kit (Colorimetric)
CD40LG
Applications/Dilutions
The human sCD40L ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sCD40L
Reactivity Notes
Human
Packaging, Storage & Formulations
Storage of components varies. See protocol for specific instructions.
Kit Components
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sCD40L
- Adhesive Films
- Human sCD40L Standard lyophilized, 20 ng/ml upon reconstitution
- HRP-Conjugate anti-human sCD40L monoclonal antibody
- Blue-Dye
- Control high, lyophilized
- Control low, lyophilized
- Green-Dye
- Dilution Plates
- Sample Diluent
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Alternate Names for Human CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)
- CD154 antigen
- CD154
- CD40 antigen ligand
- CD40 Ligand
- CD40-L
- CD40LG
- CD40LIGM
- gp39
- hCD40L
- HIGM1
- T-B cell-activating molecule
- T-BAM
- T-cell antigen Gp39
- TNF-related activation protein
- TNFSF5
- TNFSF5IMD3
- TRAP
- TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
- tumor necrosis factor (ligand) superfamily member 5
- Tumor necrosis factor ligand superfamily member 5
Background
CD40 belongs to the TNF receptor superfamily and is a biochemical marker for the identification of activated platelets of patients with cardiovascular diseases (angina pectoris, infarction, stroke etc.) A key role of CD40/CD40ligand interactions in immune activation, particularly in T-cell dependent B cell responses is anticipated. CD40 Ligand is expressed on the surface of activated CD4+ T cells, basophils, and mast cells. Binding of CD40L to its receptor, CD40, on the surface of B cells stimulates B-cell proliferation, adhesion and differentiation. A soluble isoform of CD40L has been shown to exist in the circulation. This soluble molecule is a homotrimer of a 18kDa protein exhibiting full activity in B cell proliferation and differentiation assays, is able to rescue B cells from apoptosis and binds soluble CD40. CD40L is discussed in relation to a potential role in supporting B cell tumors and it has been discovered that the molecular defect in the X-linked Hyper-IgM-Syndrome is targeted to the CD40 L gene, it is functional involved in B cell hybridomas and chronic lymphocytic leukemia as well as several autoimmune diseases.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.