Human Annexin V ELISA Kit (Colorimetric) Summary
The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human Annexin V positive serum. There was no crossreactivity detected, notably not with Annexin VIII (Vac beta).
0.78 – 50 ng/mL
0.33 ng/mL
Sandwich ELISA
11.00%
10%
The overall mean recovery was 85%. Please see protocol for more specific Spike Recovery details.
50 uL
ELISA Kit (Colorimetric)
ANXA5
Applications/Dilutions
The human Annexin V ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Annexin V.
Reactivity Notes
Human
Packaging, Storage & Formulations
Storage of components varies. See protocol for specific instructions.
Kit Components
- Streptavidin-HRP
- Substrate Solution (tetramethyl-benzidine)
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
- Stop Solution (1M Phosphoric acid)
- Sample Diluent
- Human Annexin V Standard lyophilized, 100 ng/ml upon reconstitution
- Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Annexin V
- Adhesive Films
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Biotin-Conjugate anti-human Annexin V monoclonal antibody
Alternate Names for Human Annexin V ELISA Kit (Colorimetric)
- Anchorin CII
- annexin A5
- Annexin V
- annexin-5
- ANX5
- ANXA5
- ANXV
- Calphobindin I
- Endonexin II
- ENX2CBP-I
- Lipocortin V
- PAP-I
- Placental anticoagulant protein 4
- Placental anticoagulant protein I
- PP4
- Thromboplastin inhibitor
- VAC-alpha
- Vascular anticoagulant-alpha
Background
Annexin V will bind to the phosphatidylserine-exposing apoptotic cell and can inhibit the procoagulant and proinflammatory activities of the dying cell. Total endogenous annexin V can be detected with the present ELISA which will offer further insights in the pathophysiological role of Annexin V.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.