s12909-017-0912-5

Histone H4 [Dimethyl Lys20] Antibody (2B8) Summary

    Immunogen
    Made to Human HISTONE H4
    Modification
    Dimethyl Lys20
    Specificity
    Recognizes histone H4 when it is dimethylated at K20. It does not bind to non-methylated H4. K20 is the only site on histone H4 methylated in vivo. This has been reported for use in chromatin immunoprecipitation assay(1).
    Isotype
    IgG2a
    Clonality
    Monoclonal
    Host
    Rat
    Gene
    HIST4H4
    Purity
    Protein G purified
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Applications/Dilutions

    Dilutions
        Western Blot 1:100-1:1000
        Immunoprecipitation 1:10-1:500

Reactivity Notes

Reacts with Human.

Packaging, Storage & Formulations

    Storage
    Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
    Buffer
    PBS (pH 7.4)
    Preservative
    0.09% Sodium Azide
    Concentration
    1.0 mg/ml
    Purity
    Protein G purified

Alternate Names for Histone H4 [Dimethyl Lys20] Antibody (2B8)

      H4
      H4/A
      H4/B
      H4/C
      H4/D
      H4/E
      H4/G
      H4/H
      H4/I
      H4/J
      H4/K
      H4/M
      H4/N
      H4/O
      H4/p
      H4F2
      H4FA
      H4FB
      H4FC
      H4FD
      H4FE
      H4FG
      H4FH
      H4FI
      H4FJ
      H4FK
      H4FM
      H4FN
      H4FO
      H4K20Me2
      H4M
      HIST1H4A
      HIST1H4B
      HIST1H4C
      HIST1H4D
      HIST1H4E
      HIST1H4F
      HIST1H4H
      HIST1H4I
      HIST1H4J
      HIST1H4K
      HIST1H4L
      HIST2H4
      HIST2H4A
      HIST2H4B
      HIST4H4
      histone 4, H4
      Histone Cluster 1 H4
      Histone Cluster 1 H4i
      Histone Cluster 4 H4
      histone cluster 4, H4
      Histone H4
      MGC24116

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

CCT245737

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