HLA DQ Antibody (HLADQ1) [FITC] Summary
The immunogen was the full length version of the protein: HLA-DQ (HLADQ1)
HLA-DQ (HLADQ1)
IgG1 Kappa
Monoclonal
Mouse
HLA-DQA1
Protein A or G purified
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Applications/Dilutions
- Flow Cytometry 1:10-1:1000
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one million cells in 100 microliter volume or 100 microliter of whole blood. Immunohistochemistry-paraffin/frozen. It is recommended that the reagent be titrated for optimal performance for each application.
Reactivity Notes
Human.
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
PBS (pH 7.2) and 0.2% (w/v) BSA
0.09% Sodium Azide
0.5 mg/ml
Protein A or G purified
Alternate Names for HLA DQ Antibody (HLADQ1) [FITC]
- CD
- CELIAC1DQ alpha 1 chain
- DC-1 alpha chain
- DQ-A1
- FLJ27088
- FLJ27328
- GSE
- HLA class II histocompatibility antigen, DQ(W3) alpha chain
- HLA-DCA
- HLA-DQA
- leucocyte antigen DQA1
- leukocyte antigen alpha chain
- major histocompatibility complex, class II, DQ alpha 1
- MGC149527
- MHC class II antigen
- MHC class II DQA1
- MHC class II HLA-D alpha glycoprotein
- MHC class II HLA-DQ-alpha-1
- MHC class II surface glycoprotein
- MHC HLA-DQ alpha
Background
The HLADQ1 monoclonal antibody recognizes human HLA-DQ also known as MHC class II DQ monomorphic antigen. The major histocompatability complex is composed of two heterodimeric glycoproteins ( alpha and beta chains) with apparent molecular weights of 27 and 32 kD. In contrast to other MHC class II molecules, both polypeptide chains of HLA-DQ are polymorphic, with the alpha chain showing an extremely high degree of polymorphism. HLA-DQ is expressed on B cells in the peripheral blood, weakly on activated T cells and some monocytes. HLA-DQ is absent on hematopoietic progenitors, resting T cells, erythrocytes and platelets. HLA-DQ is expressed after HLA-DR and HLA-DP in hematopoietic development. HLA-DQ presents peptide fragments mainly from degraded intravesicular and extracellular proteins to CD4+ T lymphocytes. Specific alleles of HLA-DQ have been linked to the pathogenesis of several autoimmune diseases (including diabetes), both as a susceptibility and resistance factor depending on the particular polymorphism. The HLADQ1 antibody has been reported to be useful for flow cytometric detection of human HLA-DQ and immunohistochemistry (acetone-fixed frozen tissues and formalin-fixed paraffin-embedded tissues).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.