HLA A Antibody (22.63.4) Summary

Nalm-6 cell line.

This antibody reacts with a common framework determinant of the 40-44kD protein of HLA-ABC Class I antigen. The antibody precipitates antigens of 40 – 44kD and 12kD (b2-microglobulin). Cell reactivity: The antibody reacts with all cells having HLA-ABC surface antigen. Erythrocytes and K562 and DAUDI are negative.

IgG2b

Monoclonal

Mouse

HLA-A

Protein A or G purified

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This antibody is suitable for indirect immunofluorescence analysis by flow cytometry and is an excellent positive control. It is suitable for cryostat sections but not for routine formalin fixed paraffin sections. If the tissue is prepared at 4C and fixed in PLP (Periodate-Lysine-Paraformaldehyde) before paraffin embedding, the antigen can be conserved (Hancock, 1982). SUGGESTED USAGE :DILUTION 1. Flow cytometry and indirect immunofluorescence: Dilute with isotonic buffer. Use 50 ul of diluted antibody per 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 ul buffer. 2. Indirect immunoperoxidase staining – the final dilution will depend on the assay conditions and detection system employed. However, a dilution of at least 1:15 will be applicable to most commonly used systems.

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

PBS (pH 7.4) and 50% (v/v) Glycerol

0.1% Sodium Azide

0.5 mg/ml

Protein A or G purified