journal.pone.0156150

Goat anti-Ferret IgA Secondary Antibody [Biotin] Summary

    Immunogen
    Ferret IgA a chain
    Specificity
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Ferret IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Ferret IgA and Ferret Serum.  No reaction was observed against Ferret IgM or Ferret IgG.
    Isotype
    IgG
    Clonality
    Polyclonal
    Host
    Goat
    Purity
    Immunogen affinity purified

Applications/Dilutions

    Dilutions
        Western Blot 1:2000 – 1:10000
        ELISA 1:20000 – 1:100000
        Immunohistochemistry 1:1000 – 1:5000
    Application Notes
    This product has been assayed against 1.0 ug of Ferret IgA in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:5,000 – 1:20,000 is suggested for this product.

Reactivity Notes

Reacts with Ferret.

Packaging, Storage & Formulations

    Storage
    Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA – Immunoglobulin and Protease free
    Preservative
    0.01% Sodium Azide
    Concentration
    LYOPH
    Purity
    Immunogen affinity purified
    Reconstitution Instructions
    Reconstitute with 0.1 ml deionized water.

Notes

Label Molecular Weight: 455, Restore with 1.0 mL of deionized water (or equivalent).

Alternate Names for Goat anti-Ferret IgA Secondary Antibody [Biotin]

      IgA
      Immunoglobulin A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

SNS-043

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