s41598-017-02116-1

Glucosamine (N-acetyl)-6-Sulfatase/GNS Antibody Summary

    Immunogen
    Synthetic peptides corresponding to GNS(glucosamine (N-acetyl)-6-sulfatase (Sanfilippo disease IIID)) The peptide sequence was selected from the C terminal of GNS.Peptide sequence PILRGASNLTWRSDVLVEYQGEGRNVTDPTCPSLSPGVSQCFPDCVCEDA.
    Isotype
    IgG
    Clonality
    Polyclonal
    Host
    Rabbit
    Gene
    GNS
    Purity
    Immunogen affinity purified
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Applications/Dilutions

    Dilutions
        Western Blot 1:100-1:2000
        Immunohistochemistry 1:10-1:500
    Application Notes
    This is a rabbit polyclonal antibody against GNS and was validated on Western blot.
    Theoretical MW
    58 kDa.
    Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

    Storage
    Store at -20C. Avoid freeze-thaw cycles.
    Buffer
    PBS and 2% Sucrose
    Preservative
    0.09% Sodium Azide
    Purity
    Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for Glucosamine (N-acetyl)-6-Sulfatase/GNS Antibody

      EC 3.1.6
      EC 3.1.6.14
      G6Sglucosamine-6-sulfatase
      glucosamine (N-acetyl)-6-sulfatase
      Glucosamine6Sulfatase
      Glucosamine-6-Sulfatase
      GNS
      MGC21274
      N-acetylglucosamine-6-sulfatase

Background

GNS is a lysosomal enzyme found in all cells. It is involved in the catabolism of heparin, heparin sulphate, and keratan sulphate. Deficiency of this enzyme results in the accumulation of undegraded substrate and the lysosomal storage disorder ucopolysaccharidosis type IIID (Sanfilippo D syndrome). Mucopolysaccharidosis type IIID is the least common of the four subtypes of Sanfilippo syndrome.The product of this gene is a lysosomal enzyme found in all cells. It is involved in the catabolism of heparin, heparan sulphate, and keratan sulphate. Deficiency of this enzyme results in the accumulation of undegraded substrate and the lysosomal storage disorder mucopolysaccharidosis type IIID (Sanfilippo D syndrome). Mucopolysaccharidosis type IIID is the least common of the four subtypes of Sanfilippo syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Staurosporine

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