s12884-015-0740-8

Glucagon R/GCGR Antibody Summary

    Immunogen
    Synthetic 18 amino acid peptide from C-terminus of human Glucagon Receptor.
    Epitope
    C-terminal domain of human
    Specificity
    Human Glucagon Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Isotype
    IgG
    Clonality
    Polyclonal
    Host
    Rabbit
    Gene
    GCGR
    Purity
    Immunogen affinity purified
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Applications/Dilutions

    Dilutions
        Immunohistochemistry-Paraffin assay dependent
    Positive Control Glucagon R/GCGR Lysate (NBL1-11006)

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (94%), Monkey (83%).

Packaging, Storage & Formulations

    Storage
    Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
    Buffer
    PBS
    Preservative
    0.1% Sodium Azide
    Concentration
    1.0 mg/ml
    Purity
    Immunogen affinity purified

Alternate Names for Glucagon R/GCGR Antibody

      FLJ97182
      GCGR
      GGR
      GLR
      GL-R
      Glucagon R
      glucagon receptor
      GlucagonR
      MGC138246

Background

The Glucagon Receptor mediates the effects of glucagon in controlling glucose metabolism by initiating a cascade of events that regulate the amount of glucose released from the liver into the bloodstream. Glucagon generally functions as a counterregulatory hormone, opposing the actions of insulin, in maintaining the levels of blood glucose, particularly in patients with hypoglycemia. In patients with diabetes, excess glucagon secretion plays a primary role in the metabolic perturbations associated with diabetes, such as hyperglycemia. Glucagon knockout mice exhibit reduced glood glucose levels, improved glucose tolerance, elevated blood glucagon levels, and normal insulin levels. Despite a total absence of glucagon receptors, these animals maintained near-normal glycemia and normal lipidemia, in the presence of circulating glucagon concentrations that were elevated by two orders of magnitude. A missense mutation (Gly40Ser) in the glucagon receptor gene is associated with non-insulin-dependent diabetes mellitus. The glucagon receptor has been reported to be expressed in liver and adipose, but not in adrenal medulla. ESTs have been isolated from liver/spleen and kidney libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

ASC-J10

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