FGF basic/FGF2 Antibody Summary
Recombinant full length protein (Human).
Secreted
Does not cross react with recombinant mouse and human IL6, recombinant human IL1 alpha and IL1 beta, recombinant human TNF alpha, recombinant human IL3, and recombinant human FGF acidic.
IgG
Polyclonal
Rabbit
FGF2
IgG purified
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Applications/Dilutions
- Western Blot 1:100-1:2000
- ELISA 1:100-1:2000
- Radioimmunodiffusion
Dot: Use at an assay dependent dilution. ELISA: Use at an assay dependent dilution. Neut: Use at an assay dependent dilution. RIA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 18 kDa. RIA Characterization: The antiserum is characterized utilizing a second antibody-polyethylene glycol (PEG) RIA protocol, where 0.1 ml of a minimum 1/10000 dilution of antiserum has been found to bind at least 40% of 100 picograms of iodinated recombinant human bFGF. The affinity constant (Ka) is determined by a Scatchard plot using this RIA system. Ka = minimum 1.0 x 109 L/mole.An minimum antibody concentration of 5 g/ml neutralizes 50% of the biological activity of 1 unit of bFGF using the mouse fibroblast cell line 3T3.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Reactivity Notes
Cross-reacts with Human.Not yet tested in other species.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
10mM PBS (pH 7.4)
No Preservative
IgG purified
Alternate Names for FGF basic/FGF2 Antibody
- basic fibroblast growth factor bFGF
- Basic fibroblast growth factor
- bFGF
- FGF basic
- FGF2
- FGF-2
- FGF2AS
- FGFBprostatropin
- fibroblast growth factor 2 (basic)
- GFG1
- HBGF-2
- HBGH-2
- heparin-binding growth factor 2
- NUDT6
- Prostatropin
Background
FGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
product targets : PGE synthase inhibitors