journal.pone.0177233

EMR3 Antibody (3D7) Summary

    Immunogen
    ARHO-EMR3-CD97 (EGF1) transfectants.
    Localization
    Cell membrane; Multi-pass membrane protein. EMR3 subunit alpha: Secreted, extracellular space. Note: A soluble form is also detected.
    Specificity
    NB100-65935 recognizes human epidermal growth factor (EGF) module-containing mucin-like hormone receptor 3 (EMR3), which is a 56kD member of the EGF-7 transmembrane (TM7) family of adhesion receptors. EMR3 is expressed at the cell surface as a heterodimer. The molecule is predominantly expressed on granulocytes, and at lower levels on mature myeloid cells, monocytes and dendritic cells. EMR3 is absent on lymphocytes, haematopoietic stem cells and myeloid progenitors. Studies suggest that the EMR3 molecule is up-regulated during late stages of neutrophil differentiation and is a marker for terminally differentiated cells. The exact functions of EMR3 and its ligands have not yet been determined.
    Isotype
    IgG
    Clonality
    Monoclonal
    Host
    Armenian Hamster
    Gene
    EMR3
    Purity
    Protein G purified
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Applications/Dilutions

    Dilutions
        Flow Cytometry 1:50-1:200
        Immunoprecipitation 1:10-1:500

Reactivity Notes

Reacts with Human.

Packaging, Storage & Formulations

    Storage
    Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
    Buffer
    PBS (pH 7.4)
    Preservative
    0.09% Sodium Azide
    Concentration
    1.0 mg/ml
    Purity
    Protein G purified

Alternate Names for EMR3 Antibody (3D7)

      egf-like module containing, mucin-like, hormone receptor-like 3
      EGF-like module receptor 3
      EGF-like module-containing mucin-like hormone receptor-like 3
      egf-like module-containing mucin-like receptor 3
      EMR3

Background

EMR3 is a member of the class B seven-span transmembrane (TM7) receptor family expressed predominantly by cells of the immune system. Family members are characterized by an extended extracellular region with a variable number of N-terminal epidermal growth factor (EGF)-like domains coupled to a TM7 domain via a mucin-like spacer domain. This gene is closely linked to the gene encoding egf-like molecule containing mucin-like hormone receptor 2 on chromosome 19. This protein may play a role in myeloid-myeloid interactions during immune and inflammatory responses. Alternative splicing of this gene results in two transcript variants encoding two distinct isoforms.EMR3 has been reported to be expressed in neutrophils, monocytes, and macrophages. ESTs have been isolated from liver/spleen and mixed libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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