JCDR/2016/16335.7281

Complement Component C5a R1 Antibody (20/70) Summary

    Immunogen
    RBL-2H3 transfected cells expressing murine C5aR.
    Localization
    Multi pass membrane protein.
    Specificity
    NB100-64082 recognizes murine CD88, a 45kD G-protein coupled cell surface receptor, which is otherwise known as C5aR. The CD88 molecule functions as a receptor for the complement component C5a, which is a potent proinflammatory molecule and a chemoattractant for neutrophils to sites of infection. In mouse, CD88 is expressed on granulocytes, monocytes and macrophages but not on resting or stimulated lymphocytes. Clone 20/70 has been reported to block the binding of the C5a to murine CD88.
    Isotype
    IgG2b
    Clonality
    Monoclonal
    Host
    Rat
    Gene
    C5AR1
    Purity
    Protein A or G purified
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Applications/Dilutions

    Dilutions
        Flow Cytometry 1:50-1:100

Reactivity Notes

Reacts with Mouse.

Packaging, Storage & Formulations

    Storage
    Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
    Buffer
    PBS (pH 7.4)
    Preservative
    0.09% Sodium Azide
    Concentration
    1.0 mg/ml
    Purity
    Protein A or G purified

Alternate Names for Complement Component C5a R1 Antibody (20/70)

      C5a anaphylatoxin chemotactic receptor
      C5A
      C5aR
      C5a-R
      C5AR1
      C5ARC5a anaphylatoxin receptor
      C5R1C5a ligand
      CD88 antigen
      CD88
      complement component 5 receptor 1 (C5a ligand)
      complement component 5 receptor 1
      complement component 5a receptor 1
      Complement Component C5a R1
      Complement Component C5aR1

Background

The human anaphylatoxin C5a (CD88) is a 74 residue glycopeptide which is generated by proteolytic cleavage of the complement factor C5 in the course of complement activation. A variety of biological effects evoked by C5a can be demonstrated, rendering this molecule an important mediartor of inflammation, with granulocytes and macrophages as the main target cells. All cellular responses to C5a are specifically mediated by interactions with the membrane bound C5a receptor, a seven transmembrane GTP binding coupled receptor that belongs to the rhodopsin supergene family. C5a receptor 1 expression has been reported in myeloid blood cells, brain, liver, lung, spleen, heart, kidney, and intestinal tract. ESTs have been isolated from B cell/lung/testis, nerve, and placenta libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Linsitinib

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