Collagen II Antibody [Biotin] Summary
Goats were hyperimmunized with bovine type II collagen.
Reacts with conformational determinants on bovine type II collagen as demonstrated by ELISA. May react with type II collagen from other species. Exhibits <10% cross reactivity with collagen type I, III, IV, V and VI. The antibody has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).
IgG
Polyclonal
Goat
COL2A1
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovators Reward
Applications/Dilutions
- Western Blot 1:100-1:2000
- Dot Blot 1:100-1:2000
- ELISA 1:1000-1:4000
- Immunocytochemistry/Immunofluorescence 1:10-1:20
- Immunohistochemistry 1:10-1:500
- Immunohistochemistry-Frozen 1:20-1:40
- Immunocytochemistry
- Streptavidin Full length Protein
- Streptavidin Native Protein
- Streptavidin Native Protein
Reactivity Notes
Human and Bovine.
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
PBS
Sodium Azide
Immunogen affinity purified
Alternate Names for Collagen II Antibody [Biotin]
- Alpha-1 type II collagen
- ANFH
- AOM
- cartilage collagen
- Chondrocalcin
- COL11A3
- COL2A1
- Collagen 2
- collagen alpha-1(II) chain
- Collagen II
- collagen II, alpha-1 polypeptide
- collagen type 2
- collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphysealdysplasia, congenital)
- collagen, type II, alpha 1
- Collagen-2
- MGC131516
- SEDC
Background
Collagens are highly conserved throughout evolution and are characterized by an uninterrupted “Glycine-X-Y” triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON DENATURED three dimensional epitopes. This may result in diminished reactivity of some antibodies with denatured collagen or formalin fixed, paraffin embedded tissues. Type II collagen is a fibrillar collagen found in cartilage and the vitreous humor of the eye. Collagen type II is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
product targets :　PTEN inhibitors