oc000062

Collagen I/III Antibody Summary

    Immunogen
    Bovine collagen type I and III from skin
    Specificity
    NB100-64523 reacts with both Collagen I and III. Less than 0.5% reactivity was observed with bovine albumin and immunoglobulins. Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues.
    Isotype
    IgG
    Clonality
    Polyclonal
    Host
    Rabbit
    Gene
    COL1A1
    Purity
    Immunogen affinity purified
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Applications/Dilutions

    Dilutions
        ELISA 1:100-1:2000
        Immunocytochemistry/Immunofluorescence 1:10-1:40

Reactivity Notes

Cross reacts with Bovine.

Packaging, Storage & Formulations

    Storage
    Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
    Buffer
    PBS, 1.0% Mannitol, 1.0% Dextran
    Preservative
    No Preservative
    Concentration
    LYOPH
    Purity
    Immunogen affinity purified
    Reconstitution Instructions
    Reconstitute to desired concentration.

Alternate Names for Collagen I/III Antibody

      Alpha-1 type I collagen
      Collagen 1/3
      collagen alpha 1 chain type I
      collagen alpha-1(I) chain
      collagen of skin, tendon and bone, alpha-1 chain
      collagen, type I, alpha 1
      OI4
      pro-alpha-1 collagen type 1

Background

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted “Glycine X Y” triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

BIBF 1120

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