journal.pone.0176665

Collagen I/II/III Antibody (NLI/22) Summary

    Immunogen
    Human placental collagen type 1
    Specificity
    NB100-64521 reacts with collagens type I, II and III. It does not react with human collagen types IV, V or VI, human plasma proteins or albumin from various species.
    Isotype
    IgM
    Clonality
    Monoclonal
    Host
    Mouse
    Gene
    COL1A1
    Purity
    IgM purified
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Applications/Dilutions

    Dilutions
        ELISA 1:500-1:2000
        Immunocytochemistry/Immunofluorescence 1:5-1:20

Reactivity Notes

Human. Not yet tested in other species.

Packaging, Storage & Formulations

    Storage
    Store at -20C. Avoid freeze-thaw cycles.
    Buffer
    Mannitol, Dextran and Salts
    Preservative
    No Preservative
    Concentration
    LYOPH
    Purity
    IgM purified
    Reconstitution Instructions
    Reconstitute with 1.0 ml sterilized distilled water to a final concentration of 0.1 mg/ml.

Alternate Names for Collagen I/II/III Antibody (NLI/22)

      Alpha-1 type I collagen
      collagen alpha 1 chain type I
      collagen alpha-1(I) chain
      collagen of skin, tendon and bone, alpha-1 chain
      collagen, type I, alpha 1
      OI4
      pro-alpha-1 collagen type 1

Background

Collagens are highly conserved throughout evolution and are characterised by an uninterrupted “Glycine X Y” triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

DMOG

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