Chorionic Gonadotropin beta Chain (HCG beta) Antibody (HCGb/54) – IHC-Prediluted Summary

Chorionic Gonadotropin beta Antibody (HCGb/54)

Cytoplasmic, secreted

Pregnancy & Choriocarcinoma Marker

This MAb reacts with a protein of 22kDa, identified as beta sub-unit of HCG. It does not cross react with the alpha sub-unit. HCG is a glycoprotein, which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). hCG MAb detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90% and 60% of cases respectively. 20% of lung squamous cell carcinomas are positive. hCG expression by non-trophoblastic tumors may indicate aggressive behavior.

IgG1 Kappa

Monoclonal

Mouse

CGB

Protein A or G purified

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Hu-chromosome location: 19q13.33
Immunohistochemistry-Paraffin 0.5 – 1 ug/ml; 30 min RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 – 20 min followed by cooling at RT for 20 minutes.

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Store at 4C.

10mM PBS and 0.05% BSA

0.05% Sodium Azide

Protein A or G purified