journal.pone.0143348

Chorionic Gonadotropin beta Chain (HCG beta) Antibody (HCG-60) Summary

    Immunogen
    Human chorionic gonadotropin.
    Specificity
    This antibody (clone HCG-60) reacts with beta-subunit of human chorionic gonadotropin (hCG), a 40 kDa hormone. hCG belongs to the glycoprotein hormone family together with lutropin (LH), follitropin (FSH) and thyrotropin (TSH). hCG is synthesised by trophoblastic cells of the placenta during pregnancy and stimulates the growth of corpus luteum. The HCG-60 antibody reacts with association constant 8.9 x 1010 l/mol. Following cross-reactivity (%) was determined by classic double-antibody RIA using unlabelled hormones: beta-hCG (73), alpha-hCG (1.6), hLH (1.2), hFSH (< 0.5), hTSH (< 0.5).
    Isotype
    IgG1
    Clonality
    Monoclonal
    Host
    Mouse
    Gene
    CGB
    Purity
    Protein A purified
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Applications/Dilutions

    Dilutions
        ELISA 1:2000
        Immunocytochemistry/Immunofluorescence 1:10-1:2000
    Positive Control Chorionic Gonadotropin alpha Chain (HCG alpha) Lysate (NBL1-09130)

Packaging, Storage & Formulations

    Storage
    Store at 4C. Do not freeze.
    Buffer
    PBS (pH 7.4)
    Preservative
    15mM Sodium Azide
    Concentration
    0.5 mg/ml
    Purity
    Protein A purified

Alternate Names for Chorionic Gonadotropin beta Chain (HCG beta) Antibody (HCG-60)

      CGB
      CGB3
      CGB3choriogonadotropin subunit beta
      CGB5
      CGB7
      CGB8
      CG-Beta
      Choriogonadotropin Subunit Beta
      Chorionic gonadotrophin chain beta
      chorionic gonadotropin beta 3 subunit
      Chorionic Gonadotropin beta Chain
      chorionic gonadotropin beta subunit
      chorionic gonadotropin, beta polypeptide
      hCGB

Background

Human chorionic gonadotropin (hCG) is a glycoprotein hormone produced by trophoblastic cells of the placenta beginning 10 to 12 days after conception. Maintenance of the fetus in the first trimester of pregnancy requires the production of hCG, which binds to the corpus luteum of the ovary which is stimulated to produce progesterone which in turn maintains the secretory endometrium. hCG is present only in trace amounts in non pregnant urine and sera. It rises sharply during pregnancy. HCG is composed of two non identical, non covalently linked polypeptide chains designated as the a and b subunits. The a subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH) and luteinizing hormone (LH). A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP) and HCG but negative for EMA and AFP is probably a choriocarcinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

LGK975

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