j.issn.2218-6751.2015.08.10

Ceramide Kinase Antibody Summary

    Immunogen
    Synthetic 20 amino acid peptide from N-terminal extracellular domain of human CERK.
    Epitope
    N-terminal extracellular domain of human
    Specificity
    Human CERK. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Predicted Species
    Monkey (90%), Primate (90%). Backed by our 100% Guarantee.
    Isotype
    IgG
    Clonality
    Polyclonal
    Host
    Rabbit
    Gene
    CERK
    Purity
    Immunogen affinity purified
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Applications/Dilutions

    Dilutions
        Immunohistochemistry-Paraffin 20 ug/ml
    Positive Control Ceramide Kinase Lysate (NBL1-09110)

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (90%), Gibbon (90%), Marmoset (90%), Canine (85%), Panda (85%), Bovine (80%), Elephant (80%), Rabbit (80%).

Packaging, Storage & Formulations

    Storage
    Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
    Buffer
    PBS
    Preservative
    0.1% Sodium Azide
    Concentration
    1.0 mg/ml
    Purity
    Immunogen affinity purified

Alternate Names for Ceramide Kinase Antibody

      ceramide kinase
      dA59H18.2
      DKFZp434E0211
      EC 2.7.1.138
      FLJ21430
      FLJ23239
      hCERKLipid kinase 4
      KIAA1646dA59H18.3
      lipid kinase LK4
      LK4Acylsphingosine kinase
      MGC131878

Background

CERK, which phosphorylates Cer to produce ceramide 1-phosphate (C1P), was initially described as a Ca2+-stimulated lipid kinase that copurified with brain synaptic vesicles. CERK activity has been reported in HL60 cells, mast cells, and neutrophils. CERK is involved in phagolysosome formation in polymorphonuclear leukocytes and also in liposome fusion. C1P has been reported to have mitogenic effects. More recently, C1P was found to induce arachidonic acid release and prostanoid synthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

KB-R7944 (mesylate)

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