MD.0000000000002324

Caspase-8 Antibody (C502S) Summary

    Immunogen
    Synthetic peptide derived from the amino-terminal sequence of p10 of human caspase-8.
    Isotype
    IgG1
    Clonality
    Monoclonal
    Host
    Rabbit
    Gene
    CASP8
    Purity
    Immunogen affinity purified
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Applications/Dilutions

    Dilutions
        Western Blot
        Immunoprecipitation
    Application Notes
    Western blotting (0.5-4 ug/ml) and immunoprecipitation (10 ug/ml). However, the optimal conditions should be determined individually. Detects the 10 kDa small subunit of caspase-8. The antibody does not detect the full-length of caspase-8.

Packaging, Storage & Formulations

    Storage
    Store at -20C. Avoid freeze-thaw cycles.
    Buffer
    PBS, 1.0% BSA and 50% Glycerol
    Preservative
    0.02% Thimerosal
    Purity
    Immunogen affinity purified

Alternate Names for Caspase-8 Antibody (C502S)

      AIS
      androgen receptor
      CASP8
      Caspase8
      Caspase-8
      DHTRTFM
      Dihydrotestosterone receptorHYSP1
      HUMARA
      Mch5
      NR3C4KD
      Nuclear receptor subfamily 3 group C member 4
      SMAX1SBMA
      spinal and bulbar muscular atrophy

Background

Apoptosis occurs during normal cellular development and involves dramatic changes in cellular structure. Disruption of apoptosis may contribute to cancer as well as other autoimmune diseases. Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase-8 is a 55 kDa cytosolic protein that is synthesized as an inactive pro-enzyme. Activation of caspase-8 involves a two-step proteolysis: the cleavage of caspase-8 to generate a 43 and a 12 kDa fragment which is further processed to 10 kDa. The p43 is then cleaved to yield p26 and the release of the active site containing p18.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Etrasimod

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