ajtmh.15-0904

CD8 alpha Antibody (LT8) [PE] Summary

    Immunogen
    Normal human peripheral blood lymphocytes
    Localization
    Type I membrane protein (isoform 1) and secreted (isoform 2).
    Specificity
    NB100-65399 recognises the alpha-chain of the CD8 antigen, a 32kD glycoprotein expressed by the cytotoxic-suppressor subset of T cells and weakly by NK cells. CD8 functions as a receptor for MHC Class I molecules.
    Isotype
    IgG1
    Clonality
    Monoclonal
    Host
    Mouse
    Gene
    CD8A
    Purity
    Protein A or G purified
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Applications/Dilutions

    Dilutions
        Flow Cytometry 1:10-1:1000
    Theoretical MW
    26 kDa.
    Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
    Positive Control CD8 alpha Lysate (NBL1-08980)

Reactivity Notes

Human. Not yet tested in other species.

Packaging, Storage & Formulations

    Storage
    Store at 4C. Do not freeze.
    Buffer
    PBS (pH 7.4)
    Preservative
    0.05% Sodium Azide
    Purity
    Protein A or G purified

Alternate Names for CD8 alpha Antibody (LT8) [PE]

      CD8 antigen, alpha polypeptide (p32)
      CD8
      CD8a antigen
      CD8a molecule
      Leu2 T-lymphocyte antigen
      Leu2
      MAL
      OKT8 T-cell antigen
      p32
      RPA-T8 antibody flow
      RPA-T8 Antibody
      RPA-T8 CD8
      RPA-T8 Clone
      RPA-T8 Flow
      T cell co-receptor
      T8 T-cell antigen
      T-cell antigen Leu2
      T-cell surface glycoprotein CD8 alpha chain
      T-lymphocyte differentiation antigen T8/Leu-2

Background

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

BMS-754818

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