JNEUROSCI.2715-16.2017

CD40 Ligand/TNFSF5 Antibody (MK13A4) Summary

    Immunogen
    Made to Human CD154
    Specificity
    NB100-64310 recognizes the human CD154 cell surface antigen, a 32kD glycoprotein also known as CD40 ligand. CD154 is expressed on activated T lymphocytes, predominantly CD4+ve cells and also on some basophils and mast cells. Clone MK13A4 has been reported to block binding of CD40 ligand to its receptor CD40.
    Isotype
    IgG1
    Clonality
    Monoclonal
    Host
    Mouse
    Gene
    CD40LG
    Purity
    Protein A or G purified
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Applications/Dilutions

    Dilutions
        Flow Cytometry 1:10-1:1000
        Functional
    Positive Control CD40 Ligand/TNFSF5 Lysate (NBL1-08949)

Reactivity Notes

Reacts with Human.

Packaging, Storage & Formulations

    Storage
    Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
    Buffer
    No buffer
    Preservative
    No Preservative
    Concentration
    1.0 mg/ml
    Purity
    Protein A or G purified

Alternate Names for CD40 Ligand/TNFSF5 Antibody (MK13A4)

      CD154 antigen
      CD154
      CD40 antigen ligand
      CD40 Ligand
      CD40-L
      CD40LG
      CD40LIGM
      gp39
      hCD40L
      HIGM1
      T-B cell-activating molecule
      T-BAM
      T-cell antigen Gp39
      TNF-related activation protein
      TNFSF5
      TNFSF5IMD3
      TRAP
      TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
      tumor necrosis factor (ligand) superfamily member 5
      Tumor necrosis factor ligand superfamily member 5

Background

CD40 ligand (CD40L) is a 33 kDa type II membrane glycoprotein expressed mainly on the cell surface of activated T lymphocytes, but also exists as a soluble form extracellularly. CD40L is the ligand for CD40, a member of the TNF superfamily, which is expressed on the cell surface of B cells, macrophages/monocytes, dendritic cells, vascular endothelial cells, and epithelial cells. CD40L plays an important role in B cell proliferation, antibody class switching, modulation of apoptosis in the germinal center through interaction with B cells expressing CD40, and activation of CD4+ T cells. Mutation within the CD40L gene is linked to hyper IgM syndrome, an X linked immunodeficiency disease that is characterized by elevated level of serum IgM and decreased level of other isotypes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

DBeQ

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