CCL24/Eotaxin-2/MPIF-2 Antibody (61016.11) Summary
Purified recombinant human eotaxin 2, expressed in E. coli as immunogen.
Secreted
When used as a capture in human eotaxin 2 sandwich ELISAs, this exhibits less than 0.03 % cross-reactivity with recombinant human eotaxin, MIP1 alpha, and MCP3.
IgG1
Monoclonal
Mouse
CCL24
Protein G purified
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Applications/Dilutions
- Western Blot 1-2 ug/ml
- ELISA 1:100-1:2000
ELISA: Use at a concentration of 2 ug/ml. Neut: Use at a concentration of 1 – 5 ug/ml. WB: Use at a concentration of 1 – 2 ug/ml. Predicted molecular weight: 10.6 kDa.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
PBS
No Preservative
0.5 mg/ml
Protein G purified
Alternate Names for CCL24/Eotaxin-2/MPIF-2 Antibody (61016.11)
- CCL24
- chemokine (C-C motif) ligand 24
- Ckb-6
- Eosinophil chemotactic protein 2
- Eotaxin-2
- member 24
- MPIF2
- MPIF-2
- MPIF2Myeloid progenitor inhibitory factor 2
- MPIF-2Small-inducible cytokine A24
- SCYA24CK-beta-6
Background
Eotaxin 2 is a member of the CC chemokine family, based on the presence of the CC motif and homology with other known CC chemokines. Eotaxin 2 cDNA encodes a 119 amino acid residue precursor protein with a 26 amino acid residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N glycosylation site. Mature human eotaxin 2 has a predicted molecular mass of approximately 10.6 kDa. Compared to other CC chemokines, eotaxin 2 exhibits 40 %, 42 %, and 39 % amino acid identity to MCP3, MIP1 alpha, and eotaxin, respectively. Human CC chemokine eotaxin 2 maps to chromosome 7q11.23.Both eotaxin and eotaxin 2 activate and attract eosinophils and basophils. A receptor for human eotaxin has been identified and found to be the third numbered receptor in the C-C chemokine subfamily of receptors (CCR3. On eosinophils, the effects of eotaxin 2 is inhibited by an CCR3 antibody and cross-desensitized by eotaxin and MCP4, suggesting that all three CC chemokines act through CCR3. Eotaxin 2 mRNA is weakly expressed in activated monocytes and T lymphocytes. Recombinant eotaxin 2 induces chemotaxis of eosinophils, basophils, and resting T lymphocytes but not monocytes and activated T lymphocytes. Eotaxin 2 inhibits colony formation in myleloid progenitor cells.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
product targets :　Phosphatase_Inhibitor_Cocktail_II inhibitors