C4 binding protein A Antibody (43015) Summary
Native human C4 binding protein.
Recognizes complement component 4 binding protein (C4bp). There are three variants of C4bp, composed of alpha and beta chains. The predominant form is a 570 kD complex of 7 alpha chains and 1 beta chain. The other complexes formed are a 530 kD homoheptamer of alpha chains or a 500 kD complex of 6 alpha chains and 1 beta chain. C4bp regulates the activation of the classical complement pathway. It is a cofactor to the C3b/C4b inactivator, crucial to the proteolytic cleavage of C4b and important in cleavage of C3b. C4bp also regulates the C4bC2a complex (C3 convertase), accelerating its degradation by dissociating the complement fragment C2a. Both the C4bp alpha and beta chains interact with anticoagulant protein S and C4bp alpha binds protein S. C4bp is expressed in the blood serum.
IgG2b
Monoclonal
Mouse
C4BPA
Protein A purified
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Applications/Dilutions
- ELISA 1:100 – 1:2000
- Immunohistochemistry-Paraffin 5 ug/ml
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Borate buffered saline
0.1% Sodium Azide
1.0 mg/ml
Protein A purified
Alternate Names for C4 binding protein A Antibody (43015)
- C4b binding protein, alpha chain
- C4b-binding protein alpha chain
- C4BP
- complement component 4 binding protein, alpha chain
- complement component 4 binding protein, alpha
- complement component 4-binding protein, alpha
- Proline-rich protein
- PRP
Background
C4 consists of three polypeptide chains (alpha, molecular weight 95 kDa, beta, molecular weight 75 kDa and gamma, molecular weight 33 kDa) and is usually present in serum at 200-600mg/L. It is a protein of the classical complement pathway. When activated by C1s, a peptide C4a is released and the generated C4b binds C2 which is in turn cleaved by C1s to produce the classical pathway C3 convertase, namely C4b2a. C4b also has opsonin activity and binds to macrophages and neutrophils via their CR1 receptor.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.