journal.pone.0177253

BMP-7 Antibody Summary

    Immunogen
    Synthetic peptide corresponding to the amino acids 1 – 14 of the native molecule conjugated to Keyhole limpet Haemocyanin.
    Epitope
    S-T-G-S-K-Q-R-S-Q-N-R-S-K-T
    Localization
    Secreted
    Specificity
    This product recognizes human bone morphogenetic protein 7 (BMP-7), a secreted peptide that induces bone and cartilage formation.
    Isotype
    IgG
    Clonality
    Polyclonal
    Host
    Rabbit
    Gene
    BMP7
    Purity
    IgG purified
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Applications/Dilutions

    Dilutions
        ELISA 1:20-1:200
    Positive Control BMP-7 Lysate (NBL1-08006)

Reactivity Notes

Based on sequence similarity, is expected to react with:Chicken, Chimpanzee, Dog, Mouse, Pig, Rat, Rhesus Monkey

Packaging, Storage & Formulations

    Storage
    Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
    Buffer
    PBS
    Preservative
    0.1% Sodium Azide
    Concentration
    5 mg/ml
    Purity
    IgG purified

Alternate Names for BMP-7 Antibody

      BMP7
      BMP-7
      bone morphogenetic protein 7
      Eptotermin alfa
      OP-1
      OP-1OP1
      Osteogenic protein 1

Background

Bone Morphogenetic Proteins (BMPs) are members of the TGF beta superfamily of cytokines that affect bone and cartilage formation. Similar to other TGF beta family proteins, BMPs are highly conserved across animal species. Mature BMPs are 30-38 kDa proteins that assume a TGF beta like cysteine knot configuration. Unlike TGF beta, BMPs do not form latent complexes with their propeptide counterparts. Most BMPs are homodimers, but bioactive natural heterodimers have been reported. Recently it was found that lovostatin, widely used for lowering cholesterol, also increases bone formation by turning on a gene (bmp2) that promotes local bone formation. BMPs create an environment conducive for bone marrow development by stimulating the production of specific bone matrix proteins and altering stromal cell and osteoclast proliferation. In addition to stimulating ectopic bone and cartilage development, BMPs may be an important factor in the development of the viscera, with roles in cell proliferation, apoptosis, differentiation, and morphogenesis. BMPs also appear to be responsible for normal dorsal/ventral patterning. BMPs are found in tissues that induce bone or cartilage growth, such as demineralized bone and urinary epithelium. Cellular responses to BMP5 are mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors which play significant roles in BMP binding and signaling. One BMP type II receptor and two BMP type I receptors have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Mdivi-1

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