B7-1/CD80 Antibody (1G10) [Biotin] Summary
Activated 5C2 cells
Mouse CD80 (B7-1), Mr 45-47 kDa
IgG2a Kappa
Monoclonal
Rat
CD80
Protein A or G purified
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Applications/Dilutions
- Flow Cytometry <= 1 ug/10^6 cells
- Immunoprecipitation 1:10-1:500
- In vitro assay
- Streptavidin Full length Protein
- Streptavidin Native Protein
- Streptavidin Native Protein
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
PBS
0.05% Sodium Azide
Protein A or G purified
Notes
Protect conjugated forms from light.
Alternate Names for B7-1/CD80 Antibody (1G10) [Biotin]
- Activation B7-1 antigen
- B7
- B71
- B7-1
- BB1
- CD28LG1
- CD28LGB7-1 antigen)
- CD80 antigen
- CD80 molecule
- CD80
- costimulatory factor CD80
- costimulatory molecule variant IgV-CD80
- CTLA-4 counter-receptor B7.1
- T-lymphocyte activation antigen CD80
Background
CD80 (B7-1) is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed by dendritic cells, monocytes, and peritoneal macrophages. Expression of CD80 on B cells is upregulated after activation by various means including stimulation by LPS, IL-4, and surface Ig receptor cross linking with peak expression occurring between 48 and 72 hours postactivation. (1-4) The interaction of CD80/B7-1 with its ligands, CD28 and CD152/CTLA-4, provides a potent costimulatory signal for T cells activated through the CD3/T cell receptor complex. (5, 6) The monoclonal antibody 1G10 blocks binding of CTLA-4 to CD80, but it does not block stimulation of T cells by natural antigen presenting cells. (2, 7)
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.