Asparaginase Antibody [HRP] Summary
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Asparaginase (E. coli).
Cytoplasmic and Secreted.
Recognises purified and partially purified Asparaginase (E. coli).
IgG
Polyclonal
Rabbit
ASPG
Ion exchange chromatography
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Applications/Dilutions
- Western Blot 1:1000-1:5000
- ELISA 1:10000-1:40000
- NovaLume Plus Chemiluminescent Reagent
Reactivity Notes
Cross-reacts with E. coli.Not yet tested in other species.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) – Immunoglobulin and Protease free
0.01% Gentamicin Sulfate
Ion exchange chromatography
Alternate Names for Asparaginase Antibody [HRP]
- AnsA
- AnsB
- Colaspase
- Cytoplasmic asparaginase I
- L ASNase I
- L ASNase II
- L asparaginase 1
- L asparaginase 2
- L asparaginase I
- L asparaginase II precursor
- L asparaginase II
- L asparagine amidohydrolase I
- L asparagine amidohydrolase II
Background
Asparaginase is an enzyme purified from E. coli and Erwinia carotovora. It acts by deaminating extracellular L asparagine, an amino acid that appears to be essential for protein synthesis by some tumour cells which are unable to synthesise asparagine. Asparaginase from Erwinia carotovora is serologically and biochemically distinct from asparaginase from E. coli, although its antineoplastic activity and toxicity is similar. Asparaginase is usually considered to be cell cycle phase nonspecific, but it may block some cells in G1 or S phase. Asparaginase reduces cellular and humoral immunity. E.coli contains two L asparaginase isoenzymes: L asparaginase I, a low affinity enzyme located in the cytoplasm, and L asparaginase II, a high affinity secreted enzyme.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
product targets :　Mixed Lineage Kinase inhibitors