Annexin V Antibody (VAA-33) Summary
Recombinant Annexin V
NB100-64303 specifically recognizes human Annexin V, a phospholipid binding protein which binds with high affinity and selectivity to phosphatidylserine (PS), in the presence of calcium. The translocation of PS from the cytosolic leaflet to the outer leaflet of the plasma membrane, due to a loss in plasma membrane asymmetry during apoptosis, acts as an in vivo signal for the removal of the dying cell by phagocytosis. Detection of PS using Annexin V therefore provides a reliable diagnostic tool for the indication of early programmed cell death. Annexin V has also been identified as an anticoagulant protein in the blood coagulation cascade, by acting as an inhibitor of prothrombin activation. The presence of antibodies to Annexin V, are associated with systemic lupus erythematosus (SLE), recurrent spontaneous abortions and systemic sclerosis (SSc). Clone VAA-33 has been reported to work in western blotting applications.
IgG2a
Monoclonal
Mouse
ANXA5
Protein G purified
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Applications/Dilutions
- ELISA 1:100-1:2000
- Flow Cytometry 1:10-1:1000
Reactivity Notes
Reacts with Human.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS
0.09% Sodium Azide
1.0 mg/ml
Protein G purified
Alternate Names for Annexin V Antibody (VAA-33)
- Anchorin CII
- annexin A5
- Annexin V
- annexin-5
- ANX5
- ANXA5
- ANXV
- Calphobindin I
- Endonexin II
- ENX2CBP-I
- Lipocortin V
- PAP-I
- Placental anticoagulant protein 4
- Placental anticoagulant protein I
- PP4
- Thromboplastin inhibitor
- VAC-alpha
- Vascular anticoagulant-alpha
Background
During apoptosis, phosphatidylserine (PS) is trans located from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca2+-dependent affinity for PS and therefore is used as a probe for detecting apoptosis. Annexin V has also been identified as an anticoagulant protein in the blood coagulation cascade, by acting as an inhibitor of prothrombin activation. The presence of antibodies to Annexin V is associated with systemic lupus erythematosus (SLE), recurrent spontaneous abortions and systemic sclerosis (SSc).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.